ScholarLens Back to search
2007•Chinese Journal of Analysis LaboratoryRequires access

Determination of 5 penicillin residues in pork by HPLC/MS/MS(trap)

Qi Lu, Engguli Powder

Open publisher page 0 citations

Abstract

A method was developed for the determination of 5 penicillin residues in pork by high performance liquid chromatography tandem mass spectrometry(HPLC/MS).The samples were extracted with 0.5% acetic acid,followed by homogenizing and centrifuging precipitation,cleaned-up with XAD-2 solid-phase extraction column, eluted by methanol.The separation was performed on a Agilent HC-C18(250 mm×4.6 mm i.d.,5 μm) column with a gradient system of 0.1% ammonia and acetonitrile as mobile phases.At spiked levels are 0.1、 0.2、 0.5 mg/kg,the limits of detection of the method are 1～5 ppb for the various antibiotics and recoveries of the spike experiment are from 52% to 82.8%,precisions are between 3.4% and 9.2%.The method is suitable for the determination of residues of penicillins in pork.

About this research paper

What this paper is about

A method was developed for the determination of 5 penicillin residues in pork by high performance liquid chromatography tandem mass spectrometry(HPLC/MS).The samples were extracted with 0.5% acetic acid,followed by homogenizing and centrifuging precipitation,cleaned-up with XAD-2 solid-phase extraction column, eluted by methanol.The separation was performed on a Agilent HC-C18(250 mm×4.6 mm i.d.,5 μm) column with a gradient system of 0.1% ammonia and acetonitrile as mobile phases.At spiked levels are 0.1、 0.2、 0.5 mg/kg,the limits of detection of the method are 1～5 ppb for the various antibiotics and recoveries of the spike experiment are from 52% to 82.8%,precisions are between 3.4% and 9.2%.The method is suitable for the determination of residues of penicillins in pork.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

A method was developed for the determination of 5 penicillin residues in pork by high performance liquid chromatography tandem mass spectrometry(HPLC/MS).The samples were extracted with 0.5% acetic acid,followed by homogenizing and centrifuging precipitation,cleaned-up with XAD-2 solid-phase extraction column, eluted by methanol.The separation was performed on a Agilent HC-C18(250 mm×4.6 mm i.d.,5 μm) column with a gradient system of 0.1% ammonia and acetonitrile as mobile phases.At spiked levels are 0.1、 0.2、 0.5 mg/kg,the limits of detection of the method are 1～5 ppb for the various antibiotics and recoveries of the spike experiment are from 52% to 82.8%,precisions are between 3.4% and 9.2%.The method is suitable for the determination of residues of penicillins in pork.

Key concepts: Chemistry, Chromatography, Acetic acid, Elution, High-performance liquid chromatography, Extraction (chemistry), Penicillin, Solid phase extraction

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of 5 penicillin residues in pork by HPLC/MS/MS(trap) — Research Paper | ScholarLens