Protective effects of pivanampeta on myocardial ischemia induced by isoproteronol in mice

AIM: To evaluate the protective effects of pivanampeta on myocardial ischemia induced by isoproteronol (ISO) in mice. METHODS: Forty-nine Kunming mice were divided into five groups randomly, each group including 8-11 mice and administered for 7 days. NS in control and ISO groups were given NS 10 ml·kg -1 twice a day. The three doses of pivanampeta ( 1.0, 5.0, and 25.0 mg·kg -1) in treatment groups were given by ig twice a day respectively. In the last two days, ISO was used at 20 mg·kg -1 sc half an hour after NS or pivanampeta were administered once a day in ISO and treatment groups, while NS was injected subcutaneously in control group continuously. Two hours after last subcutaneous administration of ISO or NS, the blood and hearts of mice were obtained to measure the serum levels of LDH and CK and to investigate the morphological changes of myocardium, respectively. RESULTS: Compared with the control group, the levels of LDH and CK in ISO groups were increased significantly (P 0.01), and morphologically severe injury was found in myocardial tissue and cells. And in each treatment group, the levels of LDH and CK were reversed (P 0.05), and myocardial injury reduced in comparison with that of ISO group. CONCLUSION: Pivanampeta has exact protective effect on myocardial ischemia mice induced by ISO in a dose-dependent manner.