Change of gene expression spectra of leucocyte in sepsis mice

Objective To investigate change of gene expression spectra of leucocyte in sepsis mice.Methods Fifty C56BL/6J mice were randomly divided into sepsis group(n=25)and normal group(n=25).Sepsis model was induced by administration of lipopolysaccharide(LPS,15mg/kg)through intraperitoneal injection,and was identified by pathological chnges of liver,lung,kidney and pancreas.Gene expression spectra was studied with oligonucleotide microarrays of 12489 full length mouse genes in the two groups for three times.Genes with ratio average(RA)3 or RA-3 were identified as variance genes.The relationship between variance genes and sepsis was analyzed.Results There were 591 variance genes in sepsis group,among which 354 genes were up-regulated and 257 genes down-regulated.The significantly up-regulated genes included 8 transcription factor genes,12 signal transduction genes,10 inflammatory response genes,4 immune response genes,3 cell adhesion genes,3 defense response genes,2 proteinase genes,2 endocytosis gene,4 cell apoptosis genes,5 metabolism enzyme genes,2 transport protein genes and 1 angiogenesis gene.The down-regulated genes included 2 transport protein genes,1 signal transduction gene,1 cell adhesion gene,1 cell apoptosis gene,2 defense response genes,2 transcription factor genes and 1 metabolism enzyme.gene.Conclusion Gene expression spectra in sepsis mice can be used to study the mechanism of genes in sepsis.The mechanism may be that inflammatory signals initially stimulates TPK signal pathway and G-protein-mediated signal transduction,further activates transcriptional factor of K1f5 and C/EBP β,and then promotes the target genes expression.