Effect of pretreatment temperature on hydrogen production by fermentation of anaerobic granular sludge

In order to eliminate the bacteria in inoculating sludge,which consume hydrogen,and to speed start-up as well as to rise efficiency of hydrogen production,effect of pretreatment temperatures of 65,80,95,110 and 121℃ for 30 min on hydrogen production from glucose was investigated in a batch cultures,with anaerobic granular sludge come from workshop treating wastewater of beer factory.The results come from fermentation showed that the amount of hydrogen produced,at conditions of initial pH=7.0,glucose concentration 10000 mg·L-1 and amount of inoculating sludge 2 g MLVSS·L-1,was less for untreated sludge than pretreated one.For anaerobic granular sludge treated at 95℃,the maximum accumulative hydrogen production in 120 h,133.47 ml,was obtained,and equal to hydrogen yields of 1.40 mol·mol-1 glucose,and specific hydrogen production of 15.93 mmol H2·(g MLVSS)-1.Although hydrogen production was varied for different pretreatment temperature,butyrate and acetate were dominated in soluble liquidproducts for all glucose fermentation tests,and their account was about 80% of the total volatile fatty acids and ethanol.