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2007•Journal of Shantou University Medical CollegeRequires access

Research of Poly-L-Lysine on Rat Limb Bud Cell Chondrogenic Differentiation in vitro

Cai Xiang-na

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Abstract

Objective: To study the promotion effect and possible mechanism of poly-L-lysine(PL)on rat limb bud cell chondrogenic differentiation in vitro.Methods: High density cultures of rat limb bud cell were exposed to culture media containing PL and cultured for four days.Chondrogenesis was assayed based on cartilage nodule number,Alcian blue bound(OD600)and expression of type Ⅱ collagen.By adding PL to the cultures at different time,the best time point of PL was inferred.The neural cadherin expression was examined by immunohistochemistry.Results: The best concentration of PL might be 10 μg/mL.Chondrogenesis stimulated by PL mainly happened on first 24 hours.The immunohistochemistry showed that the expressions of type Ⅱ collagen and neural cadherin were increased.Conclusion: PL can promote rat limb bud cell chondrogenic differentiation in vitro,which may be achieved by enhancing the expression of neural cadherin.

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Objective: To study the promotion effect and possible mechanism of poly-L-lysine(PL)on rat limb bud cell chondrogenic differentiation in vitro.Methods: High density cultures of rat limb bud cell were exposed to culture media containing PL and cultured for four days.Chondrogenesis was assayed based on cartilage nodule number,Alcian blue bound(OD600)and expression of type Ⅱ collagen.By adding PL to the cultures at different time,the best time point of PL was inferred.The neural cadherin expression was examined by immunohistochemistry.Results: The best concentration of PL might be 10 μg/mL.Chondrogenesis stimulated by PL mainly happened on first 24 hours.The immunohistochemistry showed that the expressions of type Ⅱ collagen and neural cadherin were increased.Conclusion: PL can promote rat limb bud cell chondrogenic differentiation in vitro,which may be achieved by enhancing the expression of neural cadherin.

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Available abstract

Objective: To study the promotion effect and possible mechanism of poly-L-lysine(PL)on rat limb bud cell chondrogenic differentiation in vitro.Methods: High density cultures of rat limb bud cell were exposed to culture media containing PL and cultured for four days.Chondrogenesis was assayed based on cartilage nodule number,Alcian blue bound(OD600)and expression of type Ⅱ collagen.By adding PL to the cultures at different time,the best time point of PL was inferred.The neural cadherin expression was examined by immunohistochemistry.Results: The best concentration of PL might be 10 μg/mL.Chondrogenesis stimulated by PL mainly happened on first 24 hours.The immunohistochemistry showed that the expressions of type Ⅱ collagen and neural cadherin were increased.Conclusion: PL can promote rat limb bud cell chondrogenic differentiation in vitro,which may be achieved by enhancing the expression of neural cadherin.

Key concepts: Chondrogenesis, Limb bud, In vitro, Cartilage, Cell biology, Cellular differentiation, Chemistry, Cell

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