A STUDY OF VASCULAR ENDOTHELIAL GROWTH FACTOR IN OVARIAN HYPERSTIMULATION SYNDROME AND ITS mRNA EXPRESSION

Objective To explore the role of vascular endothelial growth factor(VEGF) in the pathogenesis of ovarian hyperstimulation syndrome (OHSS) and its mechanism. Methods VEGF levels in 14 OHSS patients' serum collected on the day of injection of human chorionic gonadotrophin (HCG) and embryo-transfer day and in follicular fluid were measured using enzyme-labeled immunsorbent assay (ELISA), the VEGF mRNA expression in granulosa cells detected by RT-PCR and the results were compared with 22 non-OHSS patients. Results The difference of VEGF levels in serum between OHSS group and non-OHSS group on HCG injection day was not significant (t=0.97,P0.05), but there was significant difference between two groups on embryo transfer day (t=3.37, 2.19; P0.05, 0.01). There was significant difference between two groups in follicular fluid(t=3.37,2.19; P0.01,0.05). The comparison of serum VEGF levels between HCG injection day and embryo transfer day all showed significant difference in OHSS group and non-OHSS (t=50.8, 20.0; P0.01). The results of RT-PCR revealed that the expression of VEGF mRNA in OHSS group was obviously stronger than that in non-OHSS group (t=3.38, P0.01). Conclusion VEGF has some role in the pathogenesis of OHSS. Measuring VEGF levels in follicular fluid and serum on embryo transfer day can predict the occurrence of OHSS.