Expression of GST-pi and MDR1 genes in operative specimens of ovarian cancer

s] Objectives:To study the expression of GST pi and MDR1 genes in operative specimens of ovarian cancer,and to analyze the possible clinical role of GST pi and MDR1. Methods:Eighteen frozen specimens of ovarian carcinoma and ten specimens of normal ovarian tissues from patients were examined for the expression of GST pi and MDR1 genes by means of RT PCR, and quantitative analysis was performed using β actin as internal contrast.Results: Positive expression rate of GST pi and MDR1 in ovarian carcinoma were 61.1% and 33.3% ,respectively,and in contrast, 20% and 10% in normal ovarian tissues respectively. The level of GST pi gene expression in ovarian carcinoma was obviously higher than that in normal ovarian tissue (P 0.05) and MDR1 gene also had high level expression in ovarian carcinoma, but had no statistical significantance. Four patients with ovarian carcinoma had GST pi and MDR1 coexpression. Expression levels of GST pi mRNA were lower than that of protein. Conclusions: (1) GST pi and MDR1 had higher level expression in ovarian carcinoma than in normal ovarian tissues. (2) GST pi and MDR1 may have same regulating factors but different mechanisms of action. (3)Processing after transcription and/or regulation of translation level may exist in GST pi expression. [