Isolation and Identification of Swine Pseudorabies Virus

One virus was isolated from brain organ of diseased pig which came from a pig farm in Tianjin. After inoculating the virus,the white spots could be seen in chick embryo chorioallantoic membrane, and typical cytopathogenic effect(CPE)appeared in PK-15 and BHK-21 cells. TCID50 of the virus was 10-6/mL.The virus could be neutralized by standard positive serum of pseudorabies virus. After injecting the virus, rabbits showed typical pseudorabies clinical symptom and died. By the above experiment results, the isolated virus was identified as PRV.