Content Determination of Borneol Acetate in Volatile Oil from Roots of Valeriana officinalis by Gas Chromatography

[Objective] To establish the determination method of borneol acetate in volatile oil from roots of Valeriana officinalis by gas chromatography.[Method]Naphthalinum was used as the internal standard substance,the GC system consisted of capillary column(30 m × 0.25 mm,0.25 μm),5% benz-methylpolysiloxane as the stationary phase,nitrogen as the carrier gas,and FID as the detector.The detector temperature is 250 ℃,vaporizer temperature 200 ℃,column temperature 150 ℃.[Result]Both borneol acetate in volatile oil and naphthalinum had got satisfactory separation under the chromatographic condition.The accuracy was at a perfect line range between 0.393 fmg / ml and 3.146 mg / ml,the mean recovery was 97.48%,and RSD was 1.1%(n = 9).[Conclusion]The method is sensitive,accurate and reproducible,and it can be used to control the quality of volatile oil from roots of V.officinalis.