Comparison of three Non-viral vector for gene transfection

Objective:To compare the efficiency of three widely used transfection reagents.Methods:The cell line C2C12was used as host cell and plasmid pcDNA3.1+CAT containing CAT reporter gene was used as target DNA.10 4 cells per well were seeded in24-well plate and cultured for24h before transfection,and then1.5μg plasmid DNA was transfected with Ca 3 (PO 4 ) 2 ,lipofectamine2000and chitosan,which are used as vectors.The cells were hyˉdrolyzed48h after transfection by freezing and defrosting repeatedly.Relative CAT content in the cell lysate was determined by ELISA.Results:The transfection efficiency of these three non-viral vectors was evaluated on the basis of CAT content.The highest CAT content was found in the cells transfected by liposome.Conclusion:The commercial liposome has the highest transfection efficiency,chitosan,running next to liposome,is relatively better than Ca 3 (PO 4 ) 2 .