Study on Peroxidase,Superoxide Dismutase Activity and Soluble Protein Content Changes During Leaf Culture in vitro of Anthurium andraeanum
WU Guo-zhi
Abstract
WU Guo-zhi
Abstract
Changes in Peroxidase(POD),Superoxide Dismutase(SOD)activity and soluble protein content during the callus induction and buds differentiation in Anthurium andraeanum lind were studied with Guaiacol process,Nitroblue tetrazolium photoreduction method and Coomassie brilliant blue G-250 respectively.The results showed that POD,SOD and soluble protein content had a very high level in young leaves.However,there had a top level during callus induction stage.POD,SOD and soluble protein content start decrease;Activity of POD and SOD of ″Arizon″ had much higher than that of Pink Champion at the buds differentiation.After the plantlet transplanted,POD and SOD activity and soluble protein content kept low level in the two varieties since POD and SOD activity at this stage had still much higher than that of young leaves,soluble protein content had lower than that of young leaves.
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Changes in Peroxidase(POD),Superoxide Dismutase(SOD)activity and soluble protein content during the callus induction and buds differentiation in Anthurium andraeanum lind were studied with Guaiacol process,Nitroblue tetrazolium photoreduction method and Coomassie brilliant blue G-250 respectively.The results showed that POD,SOD and soluble protein content had a very high level in young leaves.However,there had a top level during callus induction stage.POD,SOD and soluble protein content start decrease;Activity of POD and SOD of ″Arizon″ had much higher than that of Pink Champion at the buds differentiation.After the plantlet transplanted,POD and SOD activity and soluble protein content kept low level in the two varieties since POD and SOD activity at this stage had still much higher than that of young leaves,soluble protein content had lower than that of young leaves.
Key concepts: Callus, Point of delivery, Peroxidase, Superoxide dismutase, Plantlet, Chemistry, Food science, Enzyme assay