Phylogenetic Diversity of Rhizobia Isolated from the Root Nodules of Peanut (Arachis hypogaea) Grown in Hebei

【Objective】 We hope to understand the diverse state of peanut rhizobia in northern part of China. 【Method】 Fifty eight rhizobial strains isolated from root nodules of peanut plants grown at different sites in Hebei Province were analyzed and compared with the reference strains of the species of Rhizobium, Agrobacterium, Sinorhizobium, Mesorhizobium and Bradyrhizobium by 16S rDNA PCR-RFLP, 16S-23S IGS PCR-RFLP and 16S rRNA gene sequencing. 【Results】 The results showed that thirty eight strains belonged Bradyrhizobium related to Bradyrhizobium japonicum and Bradyrhizobium liaoningense. 【Conclusion】 It demonstrates that peanut plants nodulate mainly with Bradyrhizobium strains in Hebei, similar to the previous reports with rhizobia from peanut plants grown in other regions of China. However, the existence of six fast-growing rhizobial strains related to R. yanglingense, R. mongolense, and R. gallicum and of ten moderately slow-growing strains indicate that peanut plants attract diverse rhizobia in Hebei Province.