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2009•Heilongjiang Medical JournalRequires access

Construction and Assessment of Gene Prokaryotic Expression Vector of Recombinant Human B Lymphocyte Cell Activating Factor

Pang Rui

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Abstract

Objective To construct human BLys gene prokaryotic expression vector. Methods To amplified total cDNA of human peripheral lymphocyte cell and to get 474 bp fragment human BLyS DNA. Then it was cut by BamHI and EcoRI enzyme and was cloned into pGEX-5X-3 which is prokaryotic expression vector. The recombinant vector was analyzed and assessed by the means of restriction analysis and DNA sequence analysis. Results BLyS cDNA has been cloned into pGEX-5X-3 correctly. Conclusion This experiment established the foundation to study the relationship between human BLys and antoimmune disease by the means to develop anti human BLyS monoclonal antibody.

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What this paper is about

Objective To construct human BLys gene prokaryotic expression vector. Methods To amplified total cDNA of human peripheral lymphocyte cell and to get 474 bp fragment human BLyS DNA. Then it was cut by BamHI and EcoRI enzyme and was cloned into pGEX-5X-3 which is prokaryotic expression vector. The recombinant vector was analyzed and assessed by the means of restriction analysis and DNA sequence analysis. Results BLyS cDNA has been cloned into pGEX-5X-3 correctly. Conclusion This experiment established the foundation to study the relationship between human BLys and antoimmune disease by the means to develop anti human BLyS monoclonal antibody.

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Available abstract

Objective To construct human BLys gene prokaryotic expression vector. Methods To amplified total cDNA of human peripheral lymphocyte cell and to get 474 bp fragment human BLyS DNA. Then it was cut by BamHI and EcoRI enzyme and was cloned into pGEX-5X-3 which is prokaryotic expression vector. The recombinant vector was analyzed and assessed by the means of restriction analysis and DNA sequence analysis. Results BLyS cDNA has been cloned into pGEX-5X-3 correctly. Conclusion This experiment established the foundation to study the relationship between human BLys and antoimmune disease by the means to develop anti human BLyS monoclonal antibody.

Key concepts: B-cell activating factor, Recombinant DNA, Complementary DNA, EcoRI, Molecular biology, Vector (molecular biology), Gene, Antibody

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