The Synthesis and Identification of Copper Complete Antigen and the Preparation of Monoclonal Antibodies against Copper Ions

[Objective] The aim was to gain the monoclonal antibody against copper ions.[Method] The carrier protein BSA,OVA were separately chelated with bifunctional chelating agent Isothiocyanobenzyl-EDTA(ITCBE) and linked with ion copper to gain the completed Immunogen(Cu-ITCBE-BSA) and the detection Immunogen(Cu-ITCBE-OVA).Non-denaturing polyacrylamide gel electrophoresis was used for the identification of the completed antigen.By using cell fusion and limiting dilution,a stable hybridoma strains secreting anti Cu2+ was obtained.[Result] A stable hybridoma strains(4A6) secreting anti Cu2+ monoclonal antibody was obtained.The sub-class of antibody was IgG1.The titer of cell culture supernatant and ascites were 1∶1024 and 1∶6.4×105 respectively.The ascites was purified by affinity chromatography,the purity was 95%.[Conclusion] A monoclonal antibody against copper ions has been obtained,which provided foundations for the detection of copper ions in environmental water samples.