The differential expression of apoptosis-associated genes in human gastric cancer MGC803 cells induced by diallyl trisulfide

Aim To investigate the differential expression of apoptosis-associated genes in human gastric cancer MGC803 cells induced by diallyl trisulfide(DATS).Methods Growth inhibition against MGC803 cells was assayed by MTT assay;The apoptosis induced by DATS was assessed by Flow cytometry and fluorescent microscope.The apoptosis-associated gene expression of MGC803 cell treated with DATS was determided by Human Apoptosis Gene Array.Apaf-1 and SODD genes were confirmed by RT-PCR.Results DATS had significant growth inhibitory activity against MGC803 cells,inhibition ratio increased from 11% to 78% at 4,8,12,16 and 24 mg·L-1 for 72 h(P0.05).The characteristic apoptotic body can be observed in partial cells under the fluorescent microscope.After treated with 8,12,16 and 24 mg·L-1 DATS for 24 h,MGC803 cells presented significant subdiploid peaks and apoptosis ratio were 23.7%,27.4% and 31.1% at average by flow cytometry,respectively(P0.05).There were 16 apoptosis-associated genes happened to express variability,in which the mRNA level of Apaf-1 was up-regulated and that of SODD was down-regulated(P0.05).Conclusions DATS-inducing apoptosis in human gastric cancer cell line MGC803 may be relevant with some specific genes and various transduction pathways.