Analysis of Antibiotics Resistance of Staphylococcus aureus Isolated from Bovine Mastitis in Shandong Province and Detection of mecA Gene

The susceptibility of 121 strains of Staphylococcus aureus isolated from bovine mastitis to six kinds of antibiotics was determined by disk diffusion method.The results showed that 26 strains occupied 21.5% were methicillin-resistant Staphylococcus aureus(MRSA),while 93 strains occupied 76.9% were susceptible to oxacillin(MSSA),and 2 strains were intermediary.There were only 2 strains of Staphylococcus aureus had the lowest resistant rate to cefotaxime,which occupied 1.6%.The PCR results showed that the mecA gene of all oxacillin-sensitive strains were negative,while that of one intermediary strain and 9 MRSA strains was positive.The MSSA strains still maintained good susceptibility to most of antibiotics,but the MRSA strains showed multi-drug resistance.PCR technology can be used as a rapid and effective way to detect the drug resistant genes of Staphylococcus aureus.