Photodynamic Therapy of Hematoporphyrin Derivative on Human Hepatocellulan Carcinoma Cell Line HepG2

To observe the effect of photodynamic therapy (PDT) on human hepatocellular carcinoma cell line HepG2 in vitro, human hepatocellular carcinoma cell lines HepG2 were studied to in vestigate the killing role of PDT with hematoporphyrin derivative (HPD) as photosensitizer and laser as light source. The cultured cells were treated with serial concentrations of HPD followed by irradiation of different dosages of laser,then MTT colorimetric assay was applied to measure the relative inhibitory rate of PDT for the cells. The apoptosis rat of human hepatic cellular carcinoma HepG2 cell lines were analyzed by flow cytometry. The relative inhibitory rate of PDT for the cells elevated along with the in crease in the concentration of sensitizer and dose of laser. Under the low photodynamic dose, it increased rapidly and gradually slowed down to reach the plateau. Under a photodynamic dose,the apoptotic rate of HepG2 cells after photodynamic therapy with HPD significantly increased,which was higher than those of the controls (P0. 01). Photodynamic therapy with HPD has a significant killing effect on human hepatocellular HepG2 cell line in vitro. The cell apoptosis may be one of the mechanisms.