2008•Zhongguo shouyi xuebaoRequires access
Establishment of infectious clone system of highly pathogenic H5N1 avian influenza virus A/Duck/Guangxi/53/02
Hualan Chen
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Abstract
A/Duck/Guangxi/53/02 is a H5N1 subtype avian influenza virus isolated in health ducks from South China,which was highly pathogenic to chickens.In our study,an eight-plasmid infectious clone system was established and the reassortant virus was rescued from cell transfection.The rescued virus(R-DKGX) maintained the same biological properties as the wild virus(W-DKGX).Both of the viruses could be recovered from the lungs of Balb/c mice 3 days after being inoculated intranasally with 106EID50.The virus titers was(3.22±0.51) lgEID50/mL and(3.69±0.88) lgEID50/mL respectively.The successful establishment of the infectious clone system of DKGX will play an important role in the studies of the molecular basis of the evolution and the relationship between the structure and function of H5N1 subtype avian influenza viruses.