Effect of benazepril on myocardial fibrosis and the expression of tumor necrosis factor-α in spontaneously hypertensive rats

Objective: To study the effect of benazepril on myocardial fibrosis and the expression of tumor necrosis factor-α(TNF-α) in spontaneously hypertensive rats(SHR).Methods: Sixteen SHRs were randomly divided into two groups:experiment group(SHR-con group) and medical intervenning group(SHR-ben group).Seven WKYs were as the normal hypertension controls(WKY group).SHR-con group were fed benazepril(10 mg·kg-1·d-1) for 12 weeks.The others groups were received distilled water.Systolic blood pressure(SBP) and diastolic blood pressure(DBP) were detected by a tube that was plugged into the carotid artery and were connected with electronic physiological polyconductor machine.Picrosirius red staining combined with computed myocardial fibrosis were employed to evaluate the collagen volume fraction(CVF),perivasular collagen area/luminal area ratio(PVCA /LA) and immunohistochemical staining evaluated TNF-α on ice slice expression in the left ventricular size tissue.Results: Compared with WKY group,the SBP,DBP,CVF,PCVA/LA and TNF-α expression(P0.01) increased markedly in SHR-con group,while these targets had decreased in SHR-ben group compared with SHR-con group.Conclusion: Chronic administration of benazepril inhibits and regresses the development of myocardial fibrosis in SHR.Except for the decreasing the blood pressure,TNF-α may be involved in the reversal mechanism of myocardial fibrosis by benazepril.