Optimization of Denaturing Gradient Gel Electrophoresis for Microbial Communities in Luzhou-flavor Daqu

Ploymerase chain reaction and denaturing gradient gel electrophoresis(PCR-DGGE) technique is used to reveal the Microbial Communities in Luzhou-flavor Daqu.In order to improve the accuracy,sensitivity and repeatability of DGGE,the running parameters of DGGE are optimized by level electrophoresis and electrophoresis time.The results show that the best range of denaturing gradients is 35%~55% of DGGE of bacteria;the best range of denaturing gradients is 30%~50% of DGGE of fungi;the best electrophoretic time is 16 hours through the time process method.The Daqu microbial community of different periods is researched by the optimized PCR-DGGE technology,and rich microbial diversity is obtained.