Construction of Survivin specific small interfering RNA expression vector,its antiproliferative and proapoptotic effect on lung adenocarcinoma cells

Objective To test the antiproliferative and proapoptotic effect of Survivin small interfering RNA(siRNA) expression vector on lung adenocarcinoma cell A549 in vitro.Methods The Survivin-siRNA expression vector was constructed and conformed by sequencing.The inhibitory effect of Survivin-siRNA was tested by fluorescent quantitative reverse transcription polymerase chain reaction(FQ RT-PCR),Western blot and immunohistochemistry.The cell proliferation and apoptotic rate were assayed by tetrazolium bromide(MTT)colorimetry and flow cytometry.Results Survivin-siRNA expression vector was constructed and transfected into A549 cell.It effectively reduced mRNA and protein level of Survivin.Immunohistochemistry also showed lower expression of Survivin.The A549 cells transfected with Survivin-siRNA had a lower cellular growth rate than that of control group(P0.01),apoptotic rate increased by 8.95% than that of control group(P0.01).Conclusion The Survivin-siRNA expression vector can inhibit the expression of Survivin gene and the proliferation of A549 cell.It can also effectively induce the apoptosis of lung adenocarcinoma cell A549.