Effects of Puerariae Lobatae Radix and Glycyrrhizae Radix et Rhizoma in Gegen Qinlian Decoction on rat liver metabolism of active ingredients in Scutellaria Radix

Objective To study the effects of Puerariae Lobatae Radix(PLR) and Glycyrrhizae Radix et Rhizoma(GRR) in Gegen Qinlian Decoction(GQD) on the rat liver microsome metabolism of baicalin,wogonoside,baicalein,and wogonin in Scutellariae Radix(SR) and to explore the compatibility mechanism of the constituents.Methods The effects of various medicinal materials in GQD,such as PLR and GRR on the rat liver microsomes metabolic activities of baicalin,wogonoside,baicalein,and wogonin were investigated by in vivo induced-in vitro liver microsomes incubation method.UPLC method was developed for the determination of baicalin,wogonoside,baicalein,and wogonin in the rat liver microsomes incubation system.Results Baicalin,wogonoside,baicalein,and wogonin were linear to be eliminated within 90,60,10,and 40 min,respectively.In the control group,the order of metabolic rate for each ingredient was baicalein wogonin wogonoside baicalin.Compared with the control group,PLR showed the metabolic inhibition on wogonoside and baicalein,but the metabolic induction on baicalin and wogonin;GRR showed the metabolic inhibition on wogonin,but the metablic induction on baicalin,wogonoside,and baicalein.Conclusion Baicalin,wogonoside,baicalein,and wogonin could be metabolized in rat liver microsomes.PLR and GRR in GQD could affect the metabolic process of the active ingredients in SR.