2000•Zhongguo shengwu huaxue yu fenzi shengwu xuebaoRequires access

Relationship between CDKs and CKIs in regulation of cell cycle progression of human gastric cancer cells

Zhengming Chen, Qiao Wu, Wen‐Jin Su

Abstract

Western blotting showed that p21~(waf1/cip1) was up-regulated by ATRA in gastric cancer cells, which resulted in a decreased CDK_(2) activity, as revealed by immunoprecipitation assay. However, p16~(ink4), which specifically inhibited cyclin D_(1)/CDK_(4) complexes, was down-regulated by ATRA both at mRNA and protein levels. By accompanying with another inhibitor p21~(waf1/cip1), it let to the increase of CDK_(4) activity after treatment of ATRA for 12 h, and the decrease after 24 h.

About this research paper

What this paper is about

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Cyclin-dependent kinase, Cell cycle, Immunoprecipitation, CDK inhibitor, Blot, Kinase, Cancer cell, Cancer research