Screening of α-Glucosidase Inhibitors From Rhizoma Anemarrhenaes by Paper Chromatography

The enzymatic reaction method,paper chromatography and spectrophotometric method were combined to screen α-glucosidase inhibitors from Rhizoma anemarrhenaes in this experiment.The experimental conditions were as follows : the concentration of substrate was 10 mg/mL,amount of the enzyme was 100 μL,and the reaction time was 8 h in enzymatic reaction;the optimum condition of paper chromatography: volume of samp1e was 70 μL,open system was n-butyl alcohol-acetone-water(7∶2∶1),developing direction was radial direction;in spectrophotometric determination,after chromatography,aniline phthalate was used as color developing reagent,then distilled water was used to elute,the wavelength for the absorbance reading was 365 nm.The experimental results show that aqueous extract,saponin,flavonoid and polysaccharide of rhizoma anemarrhenae,are good inhibitors of a-glucosidase,then Chinese herbal medicine rhizoma anemarrhenae is really excellent hypoglycemic plant resources.