Effect of IL-17 on CC Chemokine Subfamily Expression in Human Keratinocytes

Objective To explore the effect of IL-17 on CC chemokine subfamily( CCL17/TARC,CCL22/MDC,CCL11 / eotaxin and CCL27 / CTACK) expression in human keratinocytes and HaCaT cells. Methods HaCaT cells were cultured and stimulated with different concentration of IL-17( 20,40,80,160ng / mL) for 24h. Real-time RT-PCR and enzyme linked immunosorbent assay were employed to detect the mRNA levels and concentrations of CCL17,CCL22,CCL11 and CCL27 in HaCaT cells and cell-free supernatants,respectively. Results IL-17( 20,40,80,160ng / mL) could promote the mRNA expression and secretion of CCL17,CCL22 and CCL11 in cultured HaCaT cells in a dose-dependent way( all P 0. 01). There were no significant difference in CCL27 mRNA level and protein secretion between IL-17-stimulated group and control group,or among IL-17-stimulated group( all P 0. 05). Conclusion IL-17 can promote the expression of CCL17,CCL22 and CCL11 in HaCaT cells,which further substantiate the involvement of Th17 cells in the pathogenesis of atopic dermatitis.