Expression of miRNA-105 in Gastric Cancer

Objective: To investigate the miRNA expression difference between gastric cancer and nornmal gastric mucosa. Methods: Total miRNA extracted and purified from 33 pairs samples of gastric carcinoma's specimens and corresponding normal gastric tissue was tagged by fluorescent Hy3. The slides were scanned using the Axon GenePix 4000B microarray scanner.Scanned images were then imported into GenePix Pro 6.0 software(Axon) for grid alignment and data extraction. miRCURYTMLNA Array(v.16.0)(Exiqon company)was used to detect differential expression of miRNA with the sdandard of 2-fold diffrential expression. Real-time fluorescence quantitative PCR was aapplied to verify miR-105 of miRNA array results. Results: The expression of 51 miRNA was diffrent between gastric cancer and nornmal gastric mucosa.31 of them was up-regulated including miR-105, miR-548n, miR-214*, miR-4309 and etc, 20of them was down-regulated including miR-31, miR-1275, miR-26b*, miR-744 and etc. The result of real-time fluorescence quantitative PCR shows that miR-105 highly expressed in gastric cancer, and the change has great statistical significance(P0.01). Conclusions: Over expression of miRNA-105 in gastric carcinoma may be related with the pathogenesis and development of gastric cancer. Our study applied a new direction of the pathogenesis,diagnosis and treatment of gastric cancer.