Determination of cinnamyl alcohol,benaldehyde,cetophenone,cinnamic acid and cinnamaldehyde in cinnamon biocatalysis reaction by RP-HPLC

The method for determination of cinnamyl alcohol,benaldehyde,acetophenone,cinnamic acid and cinnamaldehyde in cinnamon biocatalysis reaction was established.The determination by RP-HPLC was carried out on a Kromasil-100A C18 column(250 mm×4.6 mm,5 μm)at room temperature,with acetonitrile-methanol-water-glacial acetic acid(36∶ 6∶ 58∶ 0.02)as mobile phase at 1.0 mL/min,and the detection wavelength was 205 nm.The linear range of cinnamyl alcohol is 1.03~82.16 μg/mL(r=0.999 9);the linear range of benaldehyde is 1.10~87.92 μg/mL(r=0.999 9);the linear range of acetophenone is 1.06~84.48 μg/mL(r=0.999 8);the linear range of cinnamic acid is 1.30~104.16 μg/mL(r=0.999 8);the linear range of cinnamaldehyde is 1.21~96.48 μg/mL(r=0.999 2).Average recovery of cinnamyl alcohol,benaldehyde,acetophenone,cinnamic acid and cinnamaldehyde were 99.8%,100.7%,100.6%,96.4% and 100.7%,respectively,with standard deviation of 3.4%,3.3%,2.0%,1.8% and 5.5%,respectively.