Administering a second rinse solution of low-potassium-dextran before reperfusion ameliorates the donor lung

Objective To studied the effects of administering a second rinse solution of low-potassium-dextran(LPD) before reperfusion on the donor lung of rat after long time,cold ischemic preservation.Methods Twenty-four rats were randomly divided into control group(n=12) and trial group(n=12).Donor lungs of control group were flushed with LPD solution.The levels of malonaldehyde(MDA),myeloperoxidase(MPO) and interleukin-8(IL-8) were tested in the tissue of right lung and the left lungs were reperfused with venous blood for 30 minutes.The partial pressure of oxygen(PaO_2),PaP and PawP were measured at every 10 minutes intervals during reperfusion.After reperfusion,the MDA,MPO activity and interlukin-8(IL-8) were determined in the lung tissue.Results The levels of MDA,MPO and IL-8 decreased significantly in right lung of trial group than those in control group(P0.01).After reperfusion of 20 minutes,the PaO_2 were higher in trial group than that in control group,and PaP,PawP were lower in trial group than those of control group(all P0.01).After reperfusion,the MDA,MPO and IL-8 decreased significantly in left lung of trial group than in control group(all P0.01).Conclusion A second flush with LPD solution before reperfusion can decrease the levels of MDA,MPO and IL-8,and thus improves the function of donor lungs.