Optimization on preparation of Ginkgo biloba extract-β-cyclodextrin inclusion complex by central composite design and response surface

Objective To optimize the preparation of Ginkgo biloba extract-β-cyclodextrin inclusion(GBE50-β-CD inclusion) complex by central composite design and response surface methodology.Methods Saturated aqueous solution method was used to prepare GBE50-β-CD inclusion complex.The preparation was optimized by central composite design.With the mass ratio of β-CD/ GBE50,inclusion time and temperature as the independent variables and with the inclusion rate as the dependent variable,the binomial and linear equations were fitted to the data of overall desirabilities,and the resulting equation was used to produce 3-D response surface graphs,through which optimal formulation was predicted,the best prescription process was chosen to verification test.Results It showed that the correlation coefficient of second-order quadratic model was higher and the prediction was better(r = 0.966);The optimum conditions for the preparation of GBE50-β-CD inclusion complex were as follows: the mass ratio of β-CD/GBE50 3:1,inclusion time 6 h,inclusion temperature at 60 ℃.The deviation between the result of the best craft verification test and the binomial fitting equation forecast value is 1.07%.Conclusion Central composite design and response surface methodology is successfully used to optimize the preparation of GBE50-β-CD inclusion complex.The optimized process is reliable,stable,and available for the industrial production.