Effects of Cinobufacini injections on the proliferation,apoptosis and expression of topoisomerase II of hepatocarcinoma HepG-2 cells

Objective To investigate the effects of Cinobufacini injections on the expression of topoisomeraseⅡof hepatocarcinoma HepG-2 cells.Methods Experiment were divided into control group and test group(the different Cinobufacini injections group 0.10,0.21,0.42 μg.mL-1).Cell proliferation was assessed by MTT assay.Cell cycles were shown through FCM.The expression of topoisomerase(TOPO Ⅱ) mRNA were analyzed by RT-PCR.Expressions of TOPO Ⅱprotein were analyzed by Western-Blot.Results Cinobufacini injection significantly inhibited HepG-2 cell proliferation(P 0.05) in dose and time-dependent ways,induced tumor cells arrested at the S-phase a dose dependent manner.Down regulated mRNA and protein expressions of TOPOⅡ(P 0.05) in a dose dependent manner.Conclusion Cinobufacini injections can inhibit human hepatocellular carcinoma HepG-2 cells proliferation,induced tumor cells arrested at the S-phase,regulation of TOPOⅡexpressions might be one of the mechanisms of Cinobufacini injections anti-tumor.