2012•Wuhan Daxue xuebao. Yixue banRequires access
Transduction and Effect of Heme Oxygenase-1 Mediated by Cell Penetrating Peptide PEP-1 on Myocardial Ischemia in Rats
Hai‐Xing Jiang
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Abstract
Objective: To investigate the protective effects of heme oxygenase-1(HO-1) mediated by cell penetrating peptide PEP-1 on myocardial ischemia/reperfusion(I/R) injury in rats.Methods: Fusion protein PEP-1/HO-1 was prepared.After intravenous injection of fusion protein PEP-1/HO-1,serum HO-1 was determined at different time points by ELISA to explore the best time point of injection.Eighteen male SD rats weighing 220-280 g were randomly and equally divided into three groups: control group,I/R group,and PEP-1/HO-1 + I/R group(HO group).The left anterior descending coronary artery was ligated for 30 minutes and loosed for 120 minutes to establish the model of myocardial I/R injury.After 120 minutes reperfusion,activities of serum creatine kinase(CK) and lactic dehydrogenase(LDH),and myocardial infarct size were determined.Results: HO-1 protein serum levels reached the peak level at the 1st and 3rd hours after the treatment of fusion protein PEP-1/HO-1.Compared with those respectively in control group,the activities of serum CK and LDH and myocardial infarct size increased in IR group versus those in control group,and decreased in HO group versus those in I/R group(all P0.05).Conclusion: HO-1 mediated by cell penetrating peptide PEP-1 has the protective effect on myocardial I/R injury in rats.