Sub-cloning of LRP16 gene promoter and constructing of expre ssion regulatory vectors

Objective:To explore the possible regulation m echanism of LRP16 gene expression and to clone LRP16 gene promoter molecule, sub promoter molecules and to construct sub LRP16 gene promoter pGL3 Basic vect ors Methods: A 2 7kb DNA sequence of LRP16 5′ end was obtain ed from NCBI by BLAST software. 7 different long target sequences from a healthy blood donor DNA sample were amplified by PCR amplification, then the products w ere identified by DNA sequencing and nest PCR Insert these 7 identified sub L RP16 promoter sequences into pGL3 Basic vectors. Results: All 7 LRP16 promoter sequences were successfully cloned and 7 sub LRP16 promoter pG L3 Basic vectors were well constructed. Conclusion:A known gene promoter sequence and sub promoter sequence can be freely obtained from NCBI d atabase, and this is very useful for the gene promoter cloning, sub cloning and promoter vector constructing and sub promoter vector constructing