Clone of porcine interferon-γ gene and construction of its eukaryotic expression plasmids

OBJECTIVE Cloning Porcine interferon-γ gene,re-construction of eukaryotic expression plasmid,and transfected cells.METHODS From Porcine liver tissue,genomic DNA was extracted and the interferon-γ gene was cloned by PCR amplification.Porcine interferon-γ gene was cloned into the eukaryotic expression vector PCI-neo vector and assembled into a eukaryotic expression vector PCI-neo-PoIFN-γ.The recombinant vector PCI-neo-PoIFN-γ wased transfer into Chinese hamster ovary cells (CHO) using lipofectamine the strain was screened,and screening training in the presence of G418.RESULTS A eukaryotic expression vector was constructed and transfected cell lines.CONCLUSION We have successfully cloned a Porcine interferon-γ gene and transfected cell lines.