Mutagenesis of a Hydrocarbon Degrading and Biosurfactants-Producing Bacteria with Low Energy Ions

To remediate petroleum-contaminated soil effectively,bacteria 8-11,isolated from oil contaminated soil in Daqing Oilfield,was implanted with low energy N+(90×2.6×1013 ions/cm2 and 20 keV),and finally the strain of mutant 23 which could efficiently degrade oil was obtained.The degradation of petroleum hydrocarbon by mutant 23 was investigated,and results indicated that the biodegradation rate of total petroleum hydrocarbon(TPH) reached 74% after 7d treatment,and n-alkane of oil composition was degraded completely.Biosurfactants,which produced by mutant 23 and mainly indentified as glycolipid by FT-IR analysis,could reduce the surface tension of culture from 56.1 mN/m(CK) to 29.3 mN/m.Characteristics of bacterial mutant 23 suggested its potential application in bioremediation of petroleum-contaminated soil.