2006•The Journal of ImmunologyRequires access
Construction of recombinant retrovirus particle expressing siRNA of Pokemon gene
Yijing Deng, Bing Ni, Man Jiang, Di Yang, Fan Li, Yuzhang Wu
Open publisher page 0 citations
Abstract
Objective To construct the recombinant retrovirus plasmid expressing siRNA of Pokemon gene.Methods Four sequen-(ces) corresponding to the human Pokemon gene were designed on Ambion's Web.The 4 complementary DNA fragments were synthesized,and then clone into the sites of BamHⅠ and HindⅢ of the pSilencer 5.1-H1 retrovirus vector for constructing the recombinant retrovirus plasmid.Results Recombinant retrovirus plasmid expressing siRNA of human Pokemon gene was constructed successfully.Conclusion The recombinant retrovirus plasmid expressing siRNA of Pokemon gene is constructed successfully,which could provide experimental data for RNA interference of the Pokemon gene,even provide a new idea for cancer gene therapy.