2009•Xi'nan Linxueyuan xuebaoRequires access
Cloning and sequence analysis of the FLOWERING LOCUS T homologous genes from Carya cathayensis.
Fangfang Chen, Huang You-jun, Zhengjia Wang, Liu GenHua, Jianqin Huang
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Abstract
A pair of primers with 18 bp in length were designed according to a conserved sequence of the homologous gene of different plant species FLOWERING LOCUS T(FT).Taking the genomic DNA of Carya cathayensis as template,a 152 bp long DNA fragment was amplified by polymerase chain reaction(PCR),And the fragment was cloned into a pMD19-T vector and then sequenced.The sequence analysis indicated that the deduced amino acid sequence of the fragment was part intron of the forth exon in the fragment with FT homologous genes encoding 50 amino acids,which was registered in GenBank with the accession number FJ858260.1.The homology test result showed that the deduced amino acid sequence of the fragment was 88%-96% homologous genes with FLOWERING LOCUS T(FT) of other plant species.