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Inhibitory effects of shRNA expression vector on the expression of human ovarian carcinoma Bcl-2 gene

Yu Zhang

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Abstract

Objective:To investigate the inhibitory effects of RNAi expression vector of Bcl-2 gene on Bcl-2 gene expression of SKOV3 cell of ovarian carcinoma.Methods:RNAi expression vector for Bcl-2 gene was constructed and transfected to SKOV3 cell strain of ovarian carcinoma with liposomes. RT-PCR and flow cytometer detects effect to mRNA and protein expression of Bcl-2.Results:The two kinds of constructive expression vectors inhibited both mRNA and protein expression of Bcl-2. Among Bcl-2 mRNA and protein expression of SKOV3 cell that transfected B type carrier only equaled to 32.3%of contrastive groups. The anti-rate of protein was 60.18%.Conclusion:The RNAi expression vector inhibits effectually expression of Bcl-2 gene.

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What this paper is about

Objective:To investigate the inhibitory effects of RNAi expression vector of Bcl-2 gene on Bcl-2 gene expression of SKOV3 cell of ovarian carcinoma.Methods:RNAi expression vector for Bcl-2 gene was constructed and transfected to SKOV3 cell strain of ovarian carcinoma with liposomes. RT-PCR and flow cytometer detects effect to mRNA and protein expression of Bcl-2.Results:The two kinds of constructive expression vectors inhibited both mRNA and protein expression of Bcl-2. Among Bcl-2 mRNA and protein expression of SKOV3 cell that transfected B type carrier only equaled to 32.3%of contrastive groups. The anti-rate of protein was 60.18%.Conclusion:The RNAi expression vector inhibits effectually expression of Bcl-2 gene.

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Available abstract

Objective:To investigate the inhibitory effects of RNAi expression vector of Bcl-2 gene on Bcl-2 gene expression of SKOV3 cell of ovarian carcinoma.Methods:RNAi expression vector for Bcl-2 gene was constructed and transfected to SKOV3 cell strain of ovarian carcinoma with liposomes. RT-PCR and flow cytometer detects effect to mRNA and protein expression of Bcl-2.Results:The two kinds of constructive expression vectors inhibited both mRNA and protein expression of Bcl-2. Among Bcl-2 mRNA and protein expression of SKOV3 cell that transfected B type carrier only equaled to 32.3%of contrastive groups. The anti-rate of protein was 60.18%.Conclusion:The RNAi expression vector inhibits effectually expression of Bcl-2 gene.

Key concepts: Transfection, RNA interference, Expression vector, Small hairpin RNA, Gene, Gene expression, Messenger RNA, Molecular biology

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