Establishment of human multidrug-resistant hepatocellular carcinoma cell line(7721/Adm)

AIM To establish human multidrug resistant hepatocellular carcinoma cell line (7721/Adm) and to study its characteristics. METHODS Using intermittent administration of high dose ADM, human hepatocellular carcinoma cell line (7721) was induced to human multidrug resistant hepatocellular carcinoma cell line (7721/Adm). The multidrug resistance of 7721/Adm to multianticarcinogen was detected by MTT assay, and the distribution of its cell cycle, the expressions of P gp, multidrug resistance associated protein (MRP) and GSH/GST were also detected by flow cytometry. RESULTS ①7721/Adm was resistant to many anti tumor agents. Its IC50 of ADM was 4.65 times higher than that of 7721 and its drug resistance to ADM was 2-6 times higher than that of 7721; ②The multiplication time of 7721/Adm prolonged, and the number of cells in S phase decreased while that in G1 and G2 phase increased; ③The expressions of P gp and MRP were enhanced significantly (94.6% and 69.4% respectively), but the expression of GSH/GST kept stable. CONCLUSION ①We successfully established a multidrug resistant cell line (7721/Adm) which has the basic characteristics of drug resistance cells; ②Compared with that of its parent cell line 7721, IC50 of 7721/Adm increased 4.65 times, and its multiplication time prolonged 4.6 times, drug accumulation decreased remarkably and distribution of its cell cycle changed.