Identification of rDNA ITS2 Genetic Sequence of Anopheles in Zhejiang Province

Objective To identify the vector categories of malaria transmission and provide the evidence for strategy formulation of malaria prevention and control in Zhejiang province. Methods According to the genetic characteristics of ribosomal DNA second internal transcribed spacers (ITS2), identification and comparison between the Anopheles caught from the spot in Zhejiang province and the samples of Anopheles anthropophagus, Anopheles yatsushiroensis, Anopheles sinensis, Anopheles lesteri from the lab was conducted by polymerase chain reaction (PCR). Results Amplication products from the Anopheles caught from the spot were all 250bp, which showed the identity with Anopheles sinensis from the lab. Conclusions The vector of malaria transmission found in Zhejiang province is Anopheles sinensis, with no finding of Anopheles anthropophagus from the spot.