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2009•Haiyang kexueRequires access

Construction of cDNA library from the musculature of Apostichopus japonicus

Peng Xiang

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Abstract

Total RNA of muscles of Apostichopus japonicus were extracted by TRIZOL Reagent.cDNA library was constructed with SMART cDNA library construction kit.The primary library hada high titer of 3.2×106,in which 96.7% clones were recombinant.The amplified library hada titer of 5.1×109,and the length of most cDNA inserted in the library was about 0.5~2.5 kb.It was concluded that the cDNA library of Musculature of A.japonicus had been constructed by using the SMART technique.

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What this paper is about

Total RNA of muscles of Apostichopus japonicus were extracted by TRIZOL Reagent.cDNA library was constructed with SMART cDNA library construction kit.The primary library hada high titer of 3.2×106,in which 96.7% clones were recombinant.The amplified library hada titer of 5.1×109,and the length of most cDNA inserted in the library was about 0.5~2.5 kb.It was concluded that the cDNA library of Musculature of A.japonicus had been constructed by using the SMART technique.

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Available abstract

Total RNA of muscles of Apostichopus japonicus were extracted by TRIZOL Reagent.cDNA library was constructed with SMART cDNA library construction kit.The primary library hada high titer of 3.2×106,in which 96.7% clones were recombinant.The amplified library hada titer of 5.1×109,and the length of most cDNA inserted in the library was about 0.5~2.5 kb.It was concluded that the cDNA library of Musculature of A.japonicus had been constructed by using the SMART technique.

Key concepts: Trizol, cDNA library, Complementary DNA, Apostichopus japonicus, Titer, Biology, Recombinant DNA, Molecular biology

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