The effects of Jiawei Zhishutang on the apoptosis of the intestinal mucosa in the rats with ischemia-reperfusion in the early stage

Objective To study the effects of Jiawei Zhishutang on the apoptosis of the intestinal mucosa in the rats with ischemia-reperfusion(I/R)in the early stage.Methods Fifty Wistar rats were divided into three groups randomly:control group(n=10),I/R model group(n=20)and treatment group(n=20).The I/R model of the intestinal was established by blocking and opening the superior mesenteric artery.Jiawei Zhishutang was given by intragastric administration before the operation in the treatment group.At 1st and 24 h after the operation,the changes of Bcl-2,caspase-3 and apoptosis of the epithelium mucosa were compared among the three groups.Results As compared the I/R model group,the expression of caspase-3 and apoptosis index in the intestinal epithelium mucosa were obviously reduced,and the expression level of Bcl-2 in the intestinal epithelium mucosa was obviously increased in treatment group.Conclusion Jiawei Zhishutang can obviously alleviate apoptosis of the intestinal mucosa in the rats with I/R in the early stage,and can effectively protect the function of the small intestine.