Effect of 5-Aza-2'-deoxycytidine on the expression of tumor suppressor gene MIA2 in HepG2

Objective To observe the effect of methylase inhibitor 5-Aza-2'-deoxycytidine(5-Aza-CdR) on the proliferation and apoptosis of HCC cell line HepG2 and detect the expression of MIA2 protein in HepG2 after 5-Aza-CdR treatment.Methods The proliferation and apoptosis of HepG2 treated with 5-Aza-CdR of 0,1,2,4,8,10,20 and 40 μmol/L were detected by MTT and flow cytometry assay.Then after HepG2 was treated with 0 μmol/L and 10 μmol/L of 5-Aza-CdR,the MIA2 protein expression was detected by immunohistochemistry and Western blot.Results MTT showed that after treatment with a certain concentration range of 5-Aza-CdR,the proliferation of HepG2 was inhibited and there was a positive correlation between cell proliferative inhibition rate and drug concentration.Flow cytometry showed that the apoptosis of HepG2 was increased and a positive correlation existed between early apoptosis rate and drug concentration.Immunohistochemistry and Western blot revealed that the level of MIA2 protein was elevated in 10 μmol/L group compared with that in control group(0 μmol/L).Conclusion After HepG2 is treated with 5-Aza-CdR,MIA2 protein expression is enhanced,cell proliferation is inhibited and early apoptosis is promoted.