Simultaneous Determination of Sodium Saccharin,Sesamol,Acid Orange II,Auramine O by Ultra High Performance Liquid Chromatography

An ultra high performance liquid chromatography method coupled with Photo Diode Array Detector(PDA) as detector was established for the separation and determination of 4 substances(sodium saccharin,sesamol,acid orange Ⅱ,auramine O) in flavor and fragrant samples.After extracted by 10% methanol/water solution(containing 1% ammonia V/V),the target compounds were separated on a Waters BEH C18 column(1.7μm,2.1mm×50mm) using HPLC acetonitrile and 5 mmol/L ammonium acetate aqueous solution(with 0.1%(V/V)) acetic acid) as mobile phases,and detected by a diode array detector scanned from 200 nm to 500 nm.It's exhibited that there was a good linear relationship(r0.9950) ranged from 0.5 to 20mg/L and the limits of detection was in range of 0.08—0.61mg/L.The average recoveries at three levels were in the range of 85.3%—110.3% with the relative standard deviations(RSD)less than 6%.This simple method can be used to determine 4 substances in flavor and fragrant samples simultaneously.