Effect of ionic liquid on asymmetric reduction of acetyltrimethylsilane catalyzed by immobilized Saccharomyces cerevisiae cells

Asymmetric reduction of acetyltrimethylsilane(ATMS) catalyzed by immobilized baker′s yeast(Saccharomyces cerevisiae) cells in Tris buffer-C_(4)MIm·BF_(4) co-solvent system and in Tris buffer monophasic system was comparatively studied.The effect of IL on the initial reaction rate,maximum conversion and product e.e.of the bioreduction was systematically explored.Under the optimum conditions in each medium,the initial reaction rate,maximum conversion and product e.e.were 84.8 mmol/L·h,99.2%and ≥99.9%,respectively,in Tris buffer monophasic system,while those in Tris buffer-C_(4)MIm·BF_(4) co-solvent system were 87.0 mmol/L·h,99.0% and ≥99.9%,respectively.The presence of IL in the reaction system could enhance the reaction rate,but decrease the operational stability of immobilized yeast cells.