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2012•Journal of Lanzhou UniversityRequires access

Molecular cloning and expression analysis of a novel MAP kinase gene BnMPK6 in Brassica napus

Juan Wang

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Abstract

A new MAPK cDNA BnMPK6 was isolated from Brassica napus.The cloned full-length cDNA was 1 521 bp,containing a 1185 bp ORF,a 5'-untranslated region(UTR) about 81 bp and a 3'-untranslate region(UTR) about 255 bp.The nucleotide sequence of BnMPK6 was 86.9%identical with that of AtMPK6, hence named BnMPK6(The accession NO in GenBank is HQ156228).The gene encoded a protein of 394 amino acids with a deduced molecular weight about 44.8 kDa and PI of 5.13.Analysis by real-time PCR indicated that the expression of BnMPK6 increased in response to cold stress.

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What this paper is about

A new MAPK cDNA BnMPK6 was isolated from Brassica napus.The cloned full-length cDNA was 1 521 bp,containing a 1185 bp ORF,a 5'-untranslated region(UTR) about 81 bp and a 3'-untranslate region(UTR) about 255 bp.The nucleotide sequence of BnMPK6 was 86.9%identical with that of AtMPK6, hence named BnMPK6(The accession NO in GenBank is HQ156228).The gene encoded a protein of 394 amino acids with a deduced molecular weight about 44.8 kDa and PI of 5.13.Analysis by real-time PCR indicated that the expression of BnMPK6 increased in response to cold stress.

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Available abstract

A new MAPK cDNA BnMPK6 was isolated from Brassica napus.The cloned full-length cDNA was 1 521 bp,containing a 1185 bp ORF,a 5'-untranslated region(UTR) about 81 bp and a 3'-untranslate region(UTR) about 255 bp.The nucleotide sequence of BnMPK6 was 86.9%identical with that of AtMPK6, hence named BnMPK6(The accession NO in GenBank is HQ156228).The gene encoded a protein of 394 amino acids with a deduced molecular weight about 44.8 kDa and PI of 5.13.Analysis by real-time PCR indicated that the expression of BnMPK6 increased in response to cold stress.

Key concepts: GenBank, Accession number (library science), Complementary DNA, Untranslated region, Brassica, Gene, Biology, Cloning (programming)

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Molecular cloning and expression analysis of a novel MAP kinase gene BnMPK6 in Brassica napus — Research Paper | ScholarLens