Detection of CD4~+CD25~+ regulation T cells and related cytokines in peripheral blood in SLE patients

Objective:To investigate the amount of CD4+CD25+ regulation T cells and the level of related cytokines in peripheral blood in SLE patients,and analyze the relationships between CD4+CD25+ regulation T cells and the immunopathogenesis of SLE.Methods:CD4+CD25+ regulation T cells were detected by flow cytometry in peripheral blood.Besides,related cytokines in peripheral blood were assayed by ELISA.Relativity between CD4+CD25+ regulation T cells and related cytokine was analyzed.Results:CD4+CD25+ regulation T cells were decreased obviously in peripheral blood in SLE patients compared with control group [(2.83±1.05)% vs(5.07±0.59)%,P0.05].The level of related cytokines was also decreased in SLE groups compared with control groups[IL-10:(29.48±13.69) pg/ml,vs(43.10±14.95)pg/ml;TGF-β1:(170.04±91.58) pg/ml vs(254.75±130.41)pg/ml,P0.05].There were positive relationships between CD4+CD25+ regulation T cells and related cytokine IL-10,TGF-β1 in SLE patients(r1=0.53,r2=0.64,P0.05).Conclusion:Celluar immunological function disorder is observed in SLE patients,and the abnormal expression of CD4+CD25+ T cells and related cytokines in peripheral blood may be involved in SLE immunopathogenesis.