CLONING AND SEQUENCE ANALYSIS OF cDNA ENCODING THE ANTIBACTERIAL PEPTIDE,DEFENSINA FROM THE MOSQUITO,ANOPHELES SINENSIS

Objective To clone cDNA encoding the antibacterial peptide, defensinA from mosquito Anopheles sinensis and conduct sequence analysis.Methods Total RNA was extracted from An.sinensis,primers def1 and def2 were synthesized according to literary,RNA sequence of the adult of An.sinensis was amplified by RT-PCR to get the defensin cDNA which was then cloned into T vector and sequenced.The cDNA sequence of defensin encoding gene was analysed and compared with other defensin gene of insect.Results It was found that a DNA fragment encoding defensin about 308 bp in length was obstained from An.sinensis cDNA.Its 308 bp nucleotide sequences and deduced 64 amino sequences shared highly identity with An.gambiae defensin encoding sequence(95%).It was evaluated An.sinensis defensin encoding gene is a new gene.It was given a name is An.sinensis defensisA.Conclusion An.sinensis defensinA encoding gene have been successfully cloned.These will provide for a basis to study the gene.