Study on Detection Method of Quinolones Residues in Egg

A method for determination of quinolones residues in egg by HPLC with fluorescence detector detection was established.A good linearity of the calibration curves for ciprofloxacin,enrofloxacin,sarafloxacin was obtained within the range of 2~500 ng/mL(R20.9999),and danofloxacin was obtained within the range of 0.4~100 ng/mL(R20.9999).The limit of quantification of ciprofloxacin,enrofloxacin,sarafloxacin was 10 ng/g,and danofloxacin was 2 ng/g.The average recoveries of ciprofloxacin,enrofloxacin,sarafloxacin from spiked eggs at three concentrations of 10,50,200 ng/g ranged from 70 % to 100%(n=5),and danofloxacin from spiked eggs at three concentrations of 2,10,40 ng/g ranged from 70% to 100%(n=5).The intra-and inter-batch variation coefficients were both less than 10%.