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Separation of ATP from Waste Beer Yeast Hydrolysate by SD3 Ion Exchange Resin

Dan Gan

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Abstract

The dynamic adsorption and desorption of SD3 ion exchange resin to ATP were studied. The results showed that the optimum adsorption conditions of ATP are as follows: pH of waste beer yeast hydrolysate 2.0, its concentration 1.0 mg/ml, its flow rate 8.25 g/L and diameter to height ratio of column 10 (80 g of wet resin filled into the column). The optimum eluent for removing impurity is HCl solution at pH 1.5 containing 0.05 mol/L NaCl. And the optimum elution conditions of ATP are as follows: taking NaOH solution at pH 12.0 containing 0.1 mol/L NaCl as eluent and its flow rate 0.5 ml/min.

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What this paper is about

The dynamic adsorption and desorption of SD3 ion exchange resin to ATP were studied. The results showed that the optimum adsorption conditions of ATP are as follows: pH of waste beer yeast hydrolysate 2.0, its concentration 1.0 mg/ml, its flow rate 8.25 g/L and diameter to height ratio of column 10 (80 g of wet resin filled into the column). The optimum eluent for removing impurity is HCl solution at pH 1.5 containing 0.05 mol/L NaCl. And the optimum elution conditions of ATP are as follows: taking NaOH solution at pH 12.0 containing 0.1 mol/L NaCl as eluent and its flow rate 0.5 ml/min.

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Available abstract

The dynamic adsorption and desorption of SD3 ion exchange resin to ATP were studied. The results showed that the optimum adsorption conditions of ATP are as follows: pH of waste beer yeast hydrolysate 2.0, its concentration 1.0 mg/ml, its flow rate 8.25 g/L and diameter to height ratio of column 10 (80 g of wet resin filled into the column). The optimum eluent for removing impurity is HCl solution at pH 1.5 containing 0.05 mol/L NaCl. And the optimum elution conditions of ATP are as follows: taking NaOH solution at pH 12.0 containing 0.1 mol/L NaCl as eluent and its flow rate 0.5 ml/min.

Key concepts: Hydrolysate, Chemistry, Column chromatography, Adsorption, Chromatography, Ion-exchange resin, Desorption, Elution

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