Detection of molecular cytogenetic abnormalities in 21 patients with multiple myeloma by fluorescence in situ hybridization

Objective:To investigate the molecular cytogenetic abnormalities in multiple myeloma(MM) by fluorescence in situ hybridization(FISH).Method:Twenty-one patients of MM were enrolled in this study.Panel probes of 1q21/RB1、D13S319/p53 and IgH were used to detect the 13q14 deletion,trisomy 1,p53 deletion and IgH gene rearrangements in these patients as well as conventional chromosome karyotype.Result:19 of 21 patients(90.48%) were detected to have at least one kind of molecular cytogenetic abnormalities by FISH.15 patients(71.43%) were detected to have at least more than two kinds of molecular cytogenetic abnormalities.The incidence of molecular cytogenetic abnormalities from high to low was as followed:trisomy 1(66.67%),IgH gene rearrangements(57.14%),13q14 deletion(47.62%) and p53 deletion(23.81%).3 patients(14.29%) were detected to have conventional chromosome abnormalities.The incidence of conventional chromosome abnormalities was significantly lower than that in FISH(P0.01).Conclusion:The incidence of trisomy 1,IgH gene rearrangements and 13q14 deletion was higher in MM.FISH can increase the detection sensitivity of molecular cytogenetic abnormalities in MM.